1. Equipment Name:UV-Visible Spectrophotometer
2. Equipment Details:Make: Shimadzu, Model: UV-2450
- UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules.
- Determination of hexavalent chromium (Cr VI)
Strengths
· Accurate determination
· UV-VIS spectrophotometer is the basic instrument
· Determination of maximum elements in periodic table
· Cost of maintained is low
Limitations
· Time taken for analysis
· At a time one parameter only it measures
· Colouring reagents are needed
- Mr. B. Mahender(Instrument Operator ) Office No: 040-27267007
Email Id: mahenderb@cmet.gov.in
- Dr. U.Rambabu(Characterization Incharge) Office No: 040-27267007
Email id : rambabu32@yahoo.com
Ultraviolet-Visible spectroscopy is working based on the principle of Beer Lambert’s law. It involves, absorbance is directly proportional to intensity of the colour and thickness of the medium.
Ultraviolet light: wavelengths between 190 and 400 nm
Visible light: wavelengths between 400 and 800 nm
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